產(chǎn)品展示
熒光素酶6970-00-/熒光素酶(螢火蟲)/重組型酵素/Luciferase firefly BR,98% 毫克 ,Sigma-Aldrich、Amresco、Amersham、Serva、AppliChem等品牌,
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中文名稱:
其他中文名稱: 熒光素酶(螢火蟲);重組型酵素
英文名稱: Luciferase firefly
其他英文名稱: Luciferase from Photinus pyralis (firefly)
產(chǎn)品規(guī)格: BR,98%
包裝: 毫克
:6970-00-
級別:BR
product line:BioUltra
Assay:≥98% (SDS-PAGE)
Mol wt:mol wt 20 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)
Protein:0-35% E%/280
Measuring range:≤ femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)
Foreign activity:ATPase ≤5 nanomolar units/mg protein;Nucleoside diphosphokinase ≤20 nanomolar units/mg protein
活力定義:One light unit produces a biometer peak height equivalent to 0.02 μCi of 4C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C
產(chǎn)品描述:Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively.
Note: Prior to 99, a unit of firefly luciferase activity was defined as that amount which will produce .0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0. mM D-luciferin. The former nanomolar unit is equivalent to approximay .3 x 06 light units
性狀:粉末。EC:.3.2.7
用途:生化研究。
保存:-20℃。保質(zhì)期年
品名稱 | |
英文名稱 | Luciferase firefly |
規(guī)格 | 可接受大包訂制 |
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